Infecciones postoperatorias tras la extracción de terceros molares incluidos / Postoperative infections after the extraction of impacted third molars

Las extracciones quirúrgicas de terceros molares pueden, si no son atendidas oportunamente, conllevar infecciones postoperatorias de gravedad. Este estudio se realizó sobre una población de 103 pacientes provenientes de la Provincia de Chimborazo, Ecuador con el fin de estudiar posibles infecciones postoperativa después de la extracción de terceros molares. Para ello, se realizó la estratificación de la población en función de la edad (15 a 25, 26 a 30, 31 a 40, 41 a 50 y 51 a 60 años), sexo, estudios escolares (inicial, básica, bachiller y profesional) y nivel socioecónomico (bajo, medio y alto). A la par, se estudió la presencia de bacterias altamente patógenas como la S. mutans y P. gingivalis en la cavidad bucal de las personas con infección postoperatoria. Los resultados revelaron que las molestias asociadas a la extracción de estos terceros molares son más frecuentes entre la población de 15 a 30 años, y es la población femenina la más proclive a tales inflamaciones. Los pacientes de menores recursos económicos fueron lo que acudieron a estos centros asistencias asociados al bajo costo de los mismos. Al analizar la posición del tercer molar respecto al segundo se observó una mayor frecuencia en la posición B. Asimismo, se determinó la presencia de S. mutans y P. gingivali en más de la mitad de la población tratada que debe ser atendida en el menor tiempo posible a fin de evitar males mayores. Se hace un llamado a los padres de familia y maestros para que sea orientadores de estas enfermedades(AU)

Surgical extractions of third molars can, if not treated promptly, lead to serious postoperative infections. This study was conducted on a population of 103 patients from the Province of Chimborazo, Ecuador in order to study possible postoperative infections after the extraction of third molars. For this, the population was stratified according to age (15 to 25, 26 to 30, 31 to 40, 41 to 50 and 51 to 60 years), sex, school studies (initial, basic, high school and professional) and socioeconomic level (low, medium and high). At the same time, the presence of highly pathogenic bacteria such as S. mutans and P. gingivalis in the oral cavity of people with postoperative infection was studied. The results revealed that the discomfort associated with the extraction of these third molars is more frequent among the population aged 15 to 30 years, and the female population is the most prone to such inflammation. Patients with lower economic resources were the ones who attended these assistance centers associated with their low cost. When analyzing the position of the third molar regard to the second, a higher frequency was observed in position B. Likewise, the presence of S. mutans and P. gingivali was determined in more than half of the treated population that should be treated in the minor time possible in order to avoid greater problems. A call is made to parents and teachers to guide these diseases(AU)

Comparative evaluation of antimicrobial potential of herbal extracts on Streptococcus mutans and Enterococcus faecalis An in vitro study / Avaliação comparativa do potencial antimicrobiano de extratos de ervas em Streptococcus mutans e Enterococcus faecalis: estudo in vitro

Though aloe vera extract, green tea extract and coriander oil are proven antimicrobial agents, very little information is available regarding its effects on oral bacteria, Streptococcus mutans, which is responsible for initiating caries and Enterococcus faecalis, responsible for failure of root canal treatment. Objective: To find the antimicrobial activity of aloe vera extract, black tea extract and coriander oil against S. mutans and E. faecalis. Materials and Methods: The agar well diffusion method was used to determine the antibacterial activity of Aloe vera extract, black tea extract and coriander oil. Different concentration of prepared plant extracts and coriander seed oil (50 & 100 µl) was incorporated into the wells and the plates containing S. mutans and E. faecalis were incubated at 37 °C for 24 h. The antibiotic (amoxicillin 30 µl) was used as positive control. Zone Of Inhibition (ZOI) was recorded in each plate. Results: For S. mutans, the maximum ZOI was created by coriander oil with a diameter of 25.00±0.58 mm at 50 µl and for E. faecalis, maximum ZOI was created by aloe vera extract 16.00±0.58 mm at 100 µl concentration which were far better than the control: amoxicillin 30 µl concentration. Conclusion: The extracts of Aloe vera, black tea and coriander oil, showed significant activity against the investigated microbial strains, Streptococcus mutans and Enterococcus faecalis which further helps in the development of new topical agents that help in reducing the numbers of these organisms present in the oral cavity. (AU)

Embora o extrato de aloe vera, extrato de chá verde e óleo de coentro sejam agentes antimicrobianos comprovados, há pouca informação disponível sobre seus efeitos nas bactérias orais, Streptococcus mutans, que é responsável por iniciar cáries e Enterococcus faecalis, responsável pela falha do tratamento de canal radicular. Objetivo: Avaliar a atividade antimicrobiana do extrato de aloe vera, extrato de chá preto e óleo de coentro contra S. mutans e E. faecalis. Materiais e Métodos: O método de difusão em agar foi usado para determinar a atividade antibacteriana do extrato de Aloe vera, extrato de chá preto e óleo de coentro. Diferentes concentrações dos extratos de plantas e óleo de semente de coentro (50 e 100 µl) foram preparados e colocados nos poços e nas placas contendo S. mutans e E. faecalis e foram incubadas a 37°C por 24 h. O antibiótico (amoxicilina 30 µl) foi utilizado como controle positivo. A zona de inibição (ZOI) foi registrada em cada placa. Resultados: Para S. mutans, a ZOI máxima foi obtida com o óleo de coentro com um diâmetro de 25,00 ± 0,58 mm a 50 µl e para E. faecalis, a ZOI máxima foi obtiada pelo extrato de aloe vera 16,00 ± 0,58 mm na concentração de 100 µl, as quais foram melhores do que o controle: concentração de 30 µl de amoxicilina. Conclusão: Os extratos de Aloe vera, chá preto e óleo de coentro apresentaram atividade significativa contra as cepas microbianas investigadas, Streptococcus mutans e Enterococcus faecalis auxiliando no desenvolvimento de novos agentes tópicos visando a redução do número desses organismos presentes no cavidade oral. (AU)

The Promising Efficacy of Probiotics, Casein Phosphopeptide and Casein Macropeptide as Dental Anticariogenic and Remineralizing Agents Part I; An In vitro Study

Objectives: To investigate the effect of different mixtures from nano casein phosphopeptides (CPP), amorphous calcium phosphate (ACP), probiotic Lactobacillus rhamnosus B-445 (L. rhamnosus) and casein glycomacropeptide (GMP) against Streptococcus mutans (S. mutans) growth and its adhesion. Methods: CPP was prepared from the tryptic digest of bovine casein and GMP by the action of chymosin on casein solution. Four mixtures namely: Group 1) Nano CPP; Group 2) Nano CPP+ ACP; Group 3)Nano CPP+ ACP + L. rhamnosus; and Group 4)Nano CPP+ ACP+ GMP were prepared and tested for its inhibitory activity against S. mutans growth and its adhesion to saliva-treated glass surfaces in comparison with a commercial product (GC MI paste plus) and chlorhexidine (0.2%) as a positive control.  The particle size and zeta potential of nano CPP and its complex with ACP were evaluated. Furthermore, the viability of L. rhamnosusin its mixture was determined during two weeks of storage at pH 6.8 and 8 respectively. Results: Revealed that Nano CPP had an average particle size (7.75 nm) and zeta potential (-8.43 mV) lower than that of CPP+ACP mixture. Probiotic containing mixture exhibited inhibitory activity slightly less than the positive control at pH 6.8. All tested mixtures reduced the adhesion of S. mutans to saliva-treated glass surfaces and the highest was that containing probiotic and GMP. L. rhamnosus showed acceptable stability in CPP+ACP mixture during storage period. Conclusions: All these findings suggest the use of probiotic, CPP+ACP mixture as a dental anticariogenic and remineralizing agents.

Effect of Various Agents on Oral Bacterial Phagocytosis in THP-1 Cells

Phagocytosis is a fundamental process in which phagocytes capture and ingest foreign particles including pathogenic bacteria. Several oral pathogens have anti-phagocytic strategies, which allow them to escape from and survive in phagocytes. Impaired bacteria phagocytosis increases inflammation and contributes to inflammatory diseases. The purpose of this study is to investigate the influences of various agents on oral pathogenic phagocytosis. To determine phagocytosis, Streptococcus mutans, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were stained with 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), and was measured using flowcytometery and confocal microscopy. The influencing factors on phagocytosis were evaluated through the pretreatment of ROS inhibitor (N-acetyl-L-cysteine (NAC)), lysozyme, potassium chloride (KCI) and adenosine triphosphate (ATP) in THP-1 cells. Expression of pro-inflammatory cytokines was determined by enzyme-linked immunosorbent assay (ELISA). The phagocytosis of various bacteria increased in a MOI-dependent manner. Among the tested bacteria, phagocytosis of P. gingivalis showed the highest fluorescent intensity at same infection time. Among the tested inhibitors, the NAC treatment significantly inhibited phagocytosis in all tested bacteria. In addition, NAC treatment indicated a similar pattern under the confocal microscopy. Moreover, NAC treatment significantly increased the bacteria-induced secretion of IL-1β among the tested inhibitors. Taken together, we conclude that the phagocytosis occurs differently depending on each bacterium. Down-regulation by ROS production inhibited phagocytosis and lead increased of oral pathogens-associated inflammation.

Model resin composites incorporating ZnO-NP: activity against S. mutans and physicochemical properties characterization

Abstract Although resin composites are widely used in the clinical practice, the development of recurrent caries at composite-tooth interface still remains as one of the principal shortcomings to be overcome in this field. Objectives To evaluate the activity against S. mutans biofilm of model resin composites incorporating different concentrations of ZnO-nanoparticles (ZnO-NP) and characterize their physicochemical properties. Materials and Methods Different concentrations of ZnO-NP (wt.%): E1=0, E2=0.5, E3=1, E4=2, E5=5 and E6=10 were incorporated into a model resin composite consisting of Bis-GMA-TEGDMA and barium borosilicate particles. The activity against S. mutans biofilm was evaluated by metabolic activity and lactic acid production. The following physicochemical properties were characterized: degree of conversion (DC%), flexural strength (FS), elastic modulus (EM), hardness (KHN), water sorption (Wsp), water solubility (Wsl) and translucency (TP). Results E3, E4, E5 and E6 decreased the biofilm metabolic activity and E5 and E6 decreased the lactic acid production (p<0.05). E6 presented the lowest DC% (p<0.05). No significant difference in FS and EM was found for all resin composites (p>0.05). E5 and E6 presented the lowest values of KHN (p<0.05). E6 presented a higher Wsp than E1 (p<0.05) and the highest Wsl (p<0.05). The translucency significantly decreased as the ZnO- NP concentration increased (p<0.05). Conclusions The incorporation of 2 - 5 wt.% of ZnO-NP could endow antibacterial activity to resin composites, without jeopardizing their physicochemical properties.

Inhibitory effect of 18β-glycyrrhetinic acid on the biofilm formation of Streptococcus mutans / 대한구강보건학회지

OBJECTIVES: The present study aimed at investigating the potential of using 18β-glycyrrhetinic acid against the cariogenic characteristics of Streptococcus mutans UA159. METHODS: The effects of 18β-glycyrrhetinic acid on biofilm formation and acid production were evaluated; the latter are indicators of cariogenicity of S. mutans. Biofilm architecture was also analyzed by scanning electron microscopy (SEM), and changes in gene expression related to biofilm formation were studied by quantitative RT-PCR. RESULTS: Treatment with 18β-glycyrrhetinic acid at a concentration of 20 µg/ml inhibited biofilm formation by 95% in the absence of sucrose and 60% in its presence, reduced acid production by 88.8%, and significantly suppressed the gene expression of comDE, gbpB, gtfC and vicR, which are thought to be involved in the virulence of S. mutans. CONCLUSIONS: These results suggest that 18β-glycyrrhetinic acid could be used as a complementary or alternative agent for preventing dental caries by interfering with the virulence properties of S. mutans without affecting the viability of the bacterial population.

Efecto antimicrobiano del cinamaldehído, timol, eugenol y quitosano sobre cepas de Streptococcus mutans / Antimicrobial effect of cinnamaldehyde, thymol, eugenol and chitosan on Streptococcus mutans strains

Objetivo: evaluar la actividad antimicrobiana de cinamaldehído, timol, eugenol y quitosano en comparación con clorhexidina al 0,12 por ciento sobre cepas de Streptococccus mutans. Métodos: se realizaron pruebas de susceptibilidad bacteriana de cepas de Streptococccus mutans con discos embebidos en eugenol, cinamaldehído, quitosano y timol, al 0,1 y 1 por ciento con un control positivo de clorhexidina al 0,12 por ciento evaluando los halos de inhibición existentes a las 24 h y 48 h. Resultados: el cinamaldehído al 1 por ciento mostró el mejor poder de control sobre el crecimiento de Streptococccus mutans al presentar 19,91 mm y 24,44 mm de halos de inhibición a las 24 h y 48 h, respectivamente, con una diferencia significativa de p< 0,05 entre los dos intervalos. Cuando se comparó el cinamaldehído al 1 por ciento con el control positivo de clorhexidina al 0,12 por ciento, no se encontraron diferencias significativas (p> 0,05). Conclusiones: el cinamaldehído al 1 por ciento tuvo los mejores resultados seguidos por quitosano, eugenol y timol a la misma concentración en comparación con clorhexidina al 0,12 por ciento tanto a la 24 como 48 h(AU)

Objective: to evaluate the antimicrobial activity of cinnamaldehyde, thymol, eugenol and chitosan compared to chlorhexidine-0.12 percent on Streptococcus mutans strains. Methods: bacterial susceptibility tests were performed on S. mutans strains with disks embedded in eugenol, cinnamaldehyde, chitosan and thymol, at 0.1 percent and 1 percent with a positive control of 0.12 percent chlorhexidine, evaluating existing inhibition zones after 24 and 48 hours. Results: cinnamaldehyde-1 percent showed the best control power over the growth of S. mutans, presenting 19.91 mm and 24.44 mm of inhibition halos after 24 and 48 hours, respectively, with a significant difference of p< 0.05 between the two intervals. When the cinnamaldehyde-1 percent was compared with the positive control of chlorhexidine-0.12 percent, no significant differences were found (p> 0.05). Conclusions: cinnamaldehyde-1 percent had the best results followed by chitosan, eugenol and thymol in the same concentration compared to chlorhexidine-0.12 percent after both 24 and 48 hours(AU)

AgNPs: The New Allies Against S. Mutans Biofilm - A Pilot Clinical Trial and Microbiological Assay

Abstract: The purpose of this study was to evaluate the antimicrobial properties of a new formulation containing silver nanoparticles, named Nano Silver Fluoride (NSF), to inhibit Streptococcus mutans biofilm formation on children's dental enamel. The variations in dental biofilm pH and in the Simplified-Oral-Hygiene-Index (OHI-S) also were evaluated after the treatment with NSF. This was a randomized, double-blind, crossover and prospective pilot clinical trial study in which 12 schoolchildren, aged between 7-8 years, had their dental enamel treated with two solutions: S1 - Nano Silver Fluoride and S2 - negative control (saline solution), in different experimental moments. The dental biofilm adhered to enamel treated with NSF had lower values of S. mutans viability (absorbance) and colony forming units (CFU) than the S0 (baseline) and S2. There was a statistically significant difference between the OHI-S mean values of S0 and S1. There were no differences between the biofilm pH (both before and after the use of the test substances) and among the different groups. These properties suggest that NSF has bactericidal effect against S. mutans biofilm and it may be used for clinical control and prevention of dental biofilm formation.

Resumo O objetivo do presente estudo foi avaliar as propriedades antimicrobianas de uma nova formulação composta por nanopartículas de prata, denominada nano silver fluoride (NSF), na inibição de biofilme de Streptococcus mutans sobre a superfície do esmalte dentário de crianças. Variações no pH do biofilme dental e nos valores do índice de Higiene Oral Simplificada (IHO-S) também foram avaliadas após o tratamento com NSF. Trata-se de um estudo piloto, randomizado, duplo cego, cruzado e prospectivo. A amostra foi composta por 12 crianças, de ambos os gêneros, com idades entre 7 e 8 anos, as quais utilizaram as duas soluções testes, S1- NSF e S2- controle negativo (solução salina). O biofilme dental tratado com NSF apresentou menores valores de viabilidade de S. mutans (absorbância) e de unidades formadoras de colônias (UFC) do que o biofilme baseline e o biofilme tratado com S2. Houve diferença estatisticamente significativa entre os valores médios de IHO-S dos grupos baseline e S1, com uma redução dos valores. Não houve qualquer variação nos valores de pH do biofilme dental, antes e depois do tratamento com S1 e S2 e entre os diferentes grupos. Estas propriedades sugerem que NSF possui efeito bactericida sobre o biofilme de S. mutans, podendo ser utilizado clinicamente para o controle e prevenção da formação do biofilme dentário.

Influence of Different Dentin Substrate (Caries-Affected, Caries-Infected, Sound) on Long-Term muTBS

Abstract The aim of this study was to evaluate the μTBS in different dentin substrates and water-storage periods. Twenty-four dentin blocks obtained from sound third molars were randomly divided into 3 groups: Sound dentin (Sd), Caries-affected dentin (Ca) and Caries-infected dentin (Ci). Dentin blocks from Ca and Ci groups were subjected to artificial caries development (S. mutans biofilm). The softest carious tissue was removed using spherical drills under visual inspection with Caries Detector solution (Ca group). It was considered as Ci (softer and deeply red stained dentin) and Ca (harder and slightly red stained dentin). The Adper Single Bond 2 adhesive system was applied and Z350 composite blocks were built in all groups. Teeth were stored in deionized water for 24 h at 37 ºC and sectioned into beams (1.0 mm2 section area). The beams from each tooth were randomly divided into three storages periods: 24 h, 6 months or 1 year. Specimens were submitted to µTBS using EZ test machine at a crosshead speed of 1.0 mm/min. Failure mode was examined by SEM. Data from µTBS were submitted to split plot two-way ANOVA and Tukey’s HSD tests (a=0.05). The µTBS (MPa) of Sd (41.2) was significantly higher than Ca (32.4) and Ci (27.2), regardless of storage. Ca and Ci after 6 months and 1 year, presented similar µTBS. Mixed and adhesive failures predominated in all groups. The highest µTBS values (48.1±9.1) were found for Sd at 24 h storage. Storage of specimens decreased the µTBS values for all conditions.

Resumo O objetivo neste estudo foi avaliar a resistência de união à microtração (RUµT) de um sistema adesivo convencional (Adper Single Bond 2 - SB) em diferentes substratos dentinários e períodos de armazenagem. Vinte e quatro blocos de dentina foram obtidos de terceiros molares hígidos e separados aleatoriamente em 3 grupos (n=8): dentina sadia (Ds), dentina afetada (Da) e dentina infectada (Di). A Da e a Di foram submetidas ao desenvolvimento biológico artificial de cárie (S. mutans). O tecido cariado amolecido foi removido usando broca esférica sob inspeção visual com a solução Caries Detector (grupo Da). Considerou-se como Di a dentina amolecida e fortemente pigmentada de vermelho e como Da, a dentina hígida e levemente pigmentada de vermelho. O sistema adesivo SB foi aplicado de acordo com as recomendações do fabricante e blocos da resina composta Z350 foram construídos (6 mm de altura). O conjunto (dente/bloco de resina) foi armazenado em água deionizada por 24 horas a 37 °C. Estes foram seccionados em palitos (1,0 mm2 de área), que foram separados aleatoriamente em 3 períodos de armazenagem: 24 horas, 6 meses e 1 ano. Os palitos foram submetidos ao ensaio de resistência de união à microtração na máquina EZ teste a uma velocidade de 1,0 mm/min. Dados de RUµT foram submetidos à Análise de Variância 2 fatores em esquema de parcela subdividida e ao teste de Tukey (a=0,05). Os valores de resistência (MPa) da Ds (41,2) foram significativamente maiores do que os da Da (32,4) e Di (27,2), independente do tempo de armazenagem. Di e Da, 6 meses e 1ano, apresentaram valores similares de resistência de união. As falhas adesivas e mistas foram predominantes para todos os grupos. Em conclusão, os maiores valores de RUµT (48,1±9,1) foram verificados para a Ds e 24 h de armazenagem. A armazenagem diminuiu os valores de RUµT para todas as condições.

Real-time quantitative polymerase chain reaction (QPCR) for the identification and quantification of streptococcus mutans in saliva and dental biofilm in children / Técnica de reacción de polimerasa en cadena (QPCR) en tiempo real para la identificación y cuantificación de streptococcus mutans en saliva y biopelícula dentaria de niños

ABSTRACT Introduction: the objective of this study was to use real-time qPCR to identify and quantify the Streptococcus mutans species in samples of saliva and dental biofilm. Methods: 27 children were randomly chosen with the following criteria: 8 years of age, low socio-economic levels, residing in the northern metropolitan area of Santiago de Chile; they were asked to attend an appointment while fasting with no teeth brushing for at least 12 hours, in order to collect non-stimulated saliva and a pool of supragingival dental biofilm of all the mesio-vestibular sides of anterior and posterior teeth. The amount of S. mutans in the samples was quantified by qPCR using primers that amplify a fragment of the gtfB gene of S. mutans. Results: the amplification showed 98% efficiency with a fluorescence of 3.36 cycles. The melting curve presented a single maximum at the same temperature for all samples. Conclusion: the methodology allows the specific identification and quantification of gene gtfB of S. mutans in saliva and dental biofilm in a quick and reliable manner, contributing to the identification of individual cariogenic risk.

RESUMEN. Introducción: el objetivo del presente estudio consistió en implementar la técnica de qPCR en tiempo real para identificar y cuantificar la especie Streptococcus mutans en muestras de saliva y biopelícula dentaria. Métodos: se seleccionaron al azar 27 niños de 8 años de edad, de nivel socio-económico bajo del área norte de la región metropolitana de Santiago de Chile, que se citaron en ayunas y sin cepillado durante al menos 12 horas, para colectar saliva no estimulada y un pool de biopelícula dentaria supragingival de todas las caras mesio-vestibulares de dientes anteriores y posteriores. Se cuantificó la cantidad de S. mutans en las muestras mediante qPCR empleando partidores que amplifican un fragmento del gen gtfB de S. mutans. Resultados: la amplificación presentó 98% de eficiencia con delta de fluorescencia de 3,36 ciclos. La curva de fusión (melting) presentó un solo máximo a una misma temperatura para todas las muestras. Conclusión: la metodología permite la identificación y cuantificación específica del gen gtfB de S. mutans en muestras de saliva y biopelícula dentaria, de forma rápida y exacta, aportando a la determinación del riesgo cariogénico individual.

Prevalence of Dental Caries among adolescence of Dhanusha District, Nepal

Background  and  Objectives:  In  developing  countries  like  Nepal,  the  oral  health  system  is currently  in  transition  phase  facing  a  high  prevalence  of  dental  caries.  It  has  significant  social impact in important life activities which hinders the achievement and ma intenance of good oral health  due  to  lack  of  dental  awareness  in  all  age  groups.  Streptococcus  mutans  is  the  most predominant  organism  to  cause  dental  caries.  Therefore, the  present  study  was  designed  to determine the prevalence of dental caries and to identify its etiology among adolescence.Material and Methods: This cross-sectional study was carried by following Standard protocols of Bergey’s  Manual  of  Systematic  Bacteriology  to  isolate  and  identify  the  organism.  The  data  was analyzed  using  SPPS  17.0  and  Microsoft  excels  2007  which  was  considered  as  statistically significant at p-value < 0.05.Results: The prevalence of dental caries in adolescent was found to be 33%.  The highest numbers of  patients  having  dental  caries  were  in  the  age  group  of  15-19  in  female  (66.32%)  than  male (39.70%).  Bacterial  growth  was  observed  more  in  female  (75.60%)  than  male  (24.39%)  which was found to be statistically significant (p=0.0001).  S. mutans  was found as the most important cause  of  dental  caries.  Maxillary  region  of  tooth  were  more  vulnerable  to  dental  caries  than mandibular  region  of  tooth.  Most  of  the  respondents  had  once  per  day  brushing  habit  without fluoridated dentrifice containing tooth paste.Conclusion:  The prevalence of dental caries was found one third of total.  S.mutans  was the main causes of dental caries and molars tooth are more affected than others.

Multi-Drug Resistance of Bacterial Isolates among Dental Caries Patients

Background and Objectives: Dental caries is a well known major oral health problem in most developing countries which has multifactorial etiology caused by many facultatively anaerobes. S. mutans is the main pathogen associated with this disease. Recently Multi-Drug Resistant (MDR) species of S. mutans were identified from the dental caries patients against many commercial antibiotics. MDR is a natural phenomenon, posing a serious worldwide threat to public health. Several therapeutic agents are available to treat or prevent tooth decay, but still global burden of the disease with MDR are emerging. Therefore, the present study was designed for assessing the antibiotic sensitivity pattern of commercially available antibiotics.Material and Methods: This cross-sectional study was carried out by following Standard protocols of Bergey’s Manual of Systematic Bacteriology to isolate and identify the organism and further followed by antibiotic susceptibility test of bacterial isolates by disc diffusion method.Results: Streptococcus mutans (40%) was the most predominant to cause dental caries followed by S. aureus with 28.92. Gram positive isolates were found to be frequently resistant towards penicillin and tetracycline whereas Gram negative isolates were found to be Cotrimoxazole resistant.Conclusion: A high frequency of penicillin resistance in oral isolates and its co-resistance to erythromycin, tetracycline, gentamycin and amipicillin among the pateints was observed. The various awareness programmes should be facilitating the appropriate use of antibiotic to re-establish dominance over diseases must be implemented.

Antimicrobial Effect of a Lauric Acid on Streptococcus Mutans Biofilm.

Background: The purpose of this study was to compare the antimicrobial activity of a synthetic fatty acid sodium laurate (Lauric acid) comprising dodecanoate fatty acids with chlorhexidine (CHX) or calcium hydroxide (CH) against S. mutans biofilm. Methods: S. mutans was grown on cover glass bottom dishes or human dentin disks for 3 days, and then treated with sodium laurate (20 μg/ml), non-functional fatty acid(sodium decanate, sigma Aldrich, C4151) ( (NP, 20 μg/ml), CH (20 μg/ml), 1% CHX, or saline for 5 days at 37℃. On cover glass, live and dead microbials in the biomass were measured by the Film Tracer™ Biofilm viability assay, and observed by confocal laser scanning microscopy (CLSM). On dentin disk, normal, diminished, or ruptured microbials were observed by field-emission scanning electron microscopy (FE-SEM). The results were subjected to two-tailed t-test, one-way analysis variance and post hoc test at a significance level of P=0.05. Results: Live/Dead Biofilm viability assay and CLSM demonstrated that sodium laurate treated biofilms had a significantly less bio-volume than CH, NP, and saline (P < 0.05), but had no significant difference from the CHX-treated group (P > 0.05). FE-SEM demonstrated that there was a marked decrease in aggregations of microbials and biofilm and wrinkled or ruptured microbials were frequently observed in the CHX and sodium laurate. Conclusion: Synthetic sodium laurate fatty acid exhibited significantly higher antimicrobial activity than CH by inhibiting microbial survival and biofilm growth against S. mutans, but had no significant difference compared to CHX.

The effects of lemon essential oil,limonene and tea polyphenols on cell surface hydrophobicity and adher-ence of Streptococcus mutans / 实用口腔医学杂志

Objective:To explore the effects of the natural plant ingredients lemon essential oil(LEO),limonene(LIM)and tea poly-phenols(TP)on the cell surface hydrophobicity and adherence of Streptococcus mutans(S.mutans).Methods:S.mutans were treated by sub-minimal inhibitory concentration(MIC)levels of LEO,LIMand TP respectively.Adsorption to hexadecane was used to measure the hydrophobic interaction of S.mutans.A classical 96-cell microtitre plate production assay using crystal violet staining was employed to visualize the adherence of S.mutans to hard tissue surface.Results:LEO,LIMand TP at sub-MIC levels could inhibit the cell sur-face hydrophobicity and adherence of S.mutans in a dose-dependent manner(P <0.05).At 1 /2 MIC and 1 /20 MIC,the inhibitary effect of LEO was stronger than that of LIMand TP(P <0.05).Conclusion:LEO may possess anticariogenic potential.

Inativação fotodinâmica em biofilme de Streptococcus mutans sobre bráquetes metálicos e cerâmicos: um estudo in vitro / Photodynamic inactivation of Streptococcus mutans biofilm on metal and ceramic brackets: a study in vitro

O trabalho in vitro avaliou a eficácia da inativação fotodinâmica (PDI) da eritrosina (E) e hematoporfirina IX (H), com 10 µM, utilizando LED azul, dose de 75 J/cm2 em células planctônicas e biofilme de S. mutans (UA 159). Suspensões padrões contendo107 células/mL foram preparadas e submetidas a diferentes condições experimentais: a) hematoporfirina IX e LED (H+L+); b) eritrosina eLED (E+L+); c) apenas LED (F-L+); d) tratamento somente com hematoporfirina IX (H+L-); e) somente com eritrosina (E+L-); e f) grupo controle, sem tratamento com fotossensibilizador (F) e sem a utilização de LED (F-L-). As cepas foram semeadas em ágar MSBS para contagem de unidades formadoras de colônias (UFC/mL). Na segunda parte do trabalho foi realizado a PDI em biofilme de S.mutans sobre bráquetes metálicos e cerâmicos, com H a 10 µM e LED azul. Os resultados foram submetidos à análise de variância e teste de Tukey (p<0,05) e demonstraram que a E sob efeito do LED(E+L+) não foi eficaz na PDI de células planctônicas, nos parâmetros usados (p=0,3644). No entanto, a H promoveu redução de 6,78 log10(p<0,0001), no grupo de tratamento (H+L+). A PDI com a associação da H e LED foi efetiva na redução de 100% de culturas planctônicas de S. mutans, porém o mesmo não foi observado na associação com a E, na dosimetria utilizada no experimento. A PDI no biofilme de S. mutans sobre bráquetes metálicos, com a H e LED não foi eficaz nos parâmetros utilizados (p=0,1023), no entanto, ocorreu diminuição significativa de 53% sobre bráquetes cerâmicos (p=0,004). A H IX modificada é promissora como agente fotossensibilizador a ser empregado na técnica de PDI em associação ao LED azul, sendo necessários outros ensaios, em novas concentrações e/ou dosimetrias para se conseguir a inativação bacteriana.

The in vitro study evaluated the efficacy of photodynamic inactivation(PDI) with erythrosine (E) and hematoporphyrin (H) 10 µM, using ablue light-emitting diode (LED), a fluence of 75 J/cm2, on planktoniccultures and biofilm of S. mutans (UA 159). Suspensions containing107 cells/mL were prepared and were tested under differentexperimental conditions: a) hematoporphyrin IX and LED (H+L+); b)erythrosine and LED irradiation (E+L+); c) only LED (P-L+); d)only hematoporphyrin IX (H+L-); e) only erythrosine (E+L-); and f)control group, no LED irradiation or photosensitizer (P) treatment(P-L-). After treatment, the strains were seeded onto MSBS agar inorder to determine the number of colony-forming units (CFU/mL).The second part of this work consisted of the PDI of S. mutans biofilmon metal and ceramic brackets with the H 10 μM and blue LED. Theresults were submitted to analysis of variance and the Tukey test(p<0.05) and showed that E under the effect of LED proved to beineffective in the PDI of planktonic cultures with the parameters used(p=0.3644). H, however, caused a reduction of 6.78 log10 (p<0.0001)in the treatment group (H+L+). PDI with H and LED exertedantimicrobial effect of 100% of the S. mutans strain studied, whereasthe same was not observed in the association with E in the dosimetryused in this work. PDI on S. mutans biofilm on metal brackets, with Hand LED was not effective with the parameters used (p=0.1023), however on ceramic brackets caused a significant reduction of 53%(p=0,004). Modified H IX is a promising photosensitizer to be used inthe PDI technique in combination with blue LED. Therefore, new testswith new concentrations and/or dosimetry are needed to achievebacterial inactivation.

Inativação fotodinâmica em biofilme de Streptococcus mutans sobre bráquetes metálicos e cerâmicos: um estudo in vitro / Photodynamic inactivation of Streptococcus mutans biofilm on metal and ceramic brackets: a study in vitro

O trabalho in vitro avaliou a eficácia da inativação fotodinâmica (PDI) da eritrosina (E) e hematoporfirina IX (H), com 10 µM, utilizando LED azul, dose de 75 J/cm2 em células planctônicas e biofilme de S. mutans (UA 159). Suspensões padrões contendo107 células/mL foram preparadas e submetidas a diferentes condições experimentais: a) hematoporfirina IX e LED (H+L+); b) eritrosina eLED (E+L+); c) apenas LED (F-L+); d) tratamento somente com hematoporfirina IX (H+L-); e) somente com eritrosina (E+L-); e f) grupo controle, sem tratamento com fotossensibilizador (F) e sem a utilização de LED (F-L-). As cepas foram semeadas em ágar MSBS para contagem de unidades formadoras de colônias (UFC/mL). Na segunda parte do trabalho foi realizado a PDI em biofilme de S.mutans sobre bráquetes metálicos e cerâmicos, com H a 10 µM e LED azul. Os resultados foram submetidos à análise de variância e teste de Tukey (p<0,05) e demonstraram que a E sob efeito do LED(E+L+) não foi eficaz na PDI de células planctônicas, nos parâmetros usados (p=0,3644). No entanto, a H promoveu redução de 6,78 log10(p<0,0001), no grupo de tratamento (H+L+). A PDI com a associação da H e LED foi efetiva na redução de 100% de culturas planctônicas de S. mutans, porém o mesmo não foi observado na associação com a E, na dosimetria utilizada no experimento. A PDI no biofilme de S. mutans sobre bráquetes metálicos, com a H e LED não foi eficaz nos parâmetros utilizados (p=0,1023), no entanto, ocorreu diminuição significativa de 53% sobre bráquetes cerâmicos (p=0,004). A H IX modificada é promissora como agente fotossensibilizador a ser empregado na técnica de PDI em associação ao LED azul, sendo necessários outros ensaios, em novas concentrações e/ou dosimetrias para se conseguir a inativação bacteriana

The in vitro study evaluated the efficacy of photodynamic inactivation(PDI) with erythrosine (E) and hematoporphyrin (H) 10 µM, using ablue light-emitting diode (LED), a fluence of 75 J/cm2, on planktoniccultures and biofilm of S. mutans (UA 159). Suspensions containing107 cells/mL were prepared and were tested under differentexperimental conditions: a) hematoporphyrin IX and LED (H+L+); b)erythrosine and LED irradiation (E+L+); c) only LED (P-L+); d)only hematoporphyrin IX (H+L-); e) only erythrosine (E+L-); and f)control group, no LED irradiation or photosensitizer (P) treatment(P-L-). After treatment, the strains were seeded onto MSBS agar inorder to determine the number of colony-forming units (CFU/mL).The second part of this work consisted of the PDI of S. mutans biofilmon metal and ceramic brackets with the H 10 μM and blue LED. Theresults were submitted to analysis of variance and the Tukey test(p<0.05) and showed that E under the effect of LED proved to beineffective in the PDI of planktonic cultures with the parameters used(p=0.3644). H, however, caused a reduction of 6.78 log10 (p<0.0001)in the treatment group (H+L+). PDI with H and LED exertedantimicrobial effect of 100% of the S. mutans strain studied, whereasthe same was not observed in the association with E in the dosimetryused in this work. PDI on S. mutans biofilm on metal brackets, with Hand LED was not effective with the parameters used (p=0.1023), however on ceramic brackets caused a significant reduction of 53%(p=0,004). Modified H IX is a promising photosensitizer to be used inthe PDI technique in combination with blue LED. Therefore, new testswith new concentrations and/or dosimetry are needed to achievebacterial inactivation

In vitro biosynthesis of autoinducer 2 of Streptococcus mutans UA1 59 and observation of the influence factors / 实用口腔医学杂志

Objective:To synthesize autoinducer-2 by the clone and prokaryotic expression of Streptococcus mutans(S.mutans)UAl59 luxS gene and to observe the influence factors.Methods:The expression vector pET21 a(+)-luxS of S.mutans UAl59 was transformed into Escheriehia coli BL2l(DE3).The S-ribosylhomocysteinase(Luxs)expression was induced by IPTG.The His tag fusion protein was isolated by Ni-chelating column and identified by Western blotting.Finally the protein was renatured by dialysis method.S-ribosylhomo-cysteine (SAH)was catalyzed by s-adenosylhomocysteine nucleosidas (Pfs)and LuxS,and then AI-2 was syntheszed.The AI-2 activi-ty was examined by luminescence of Vibrio harveyi BB1 70 when the concentration of LuxS protein or pH(4 -1 2)or the concentration of sodium fluoride was changed in reaction mixes of AI-2 synthesis.Results:Compared with the control group,with the increase of LuxS protein concentration,the relative activity of in vitro synthesized AI-2 increased gradually(P <0.001 ).When pH was between 6 -1 0, the relative activity of AI-2 were the highest,beyond the range of pH,the relative activity of AI-2 decreased(P <0.001 ).When a final concentration of sodium fluoride was more than 0.3%,the luminescence values decreased(P <0.05).Conclusion:LuxS fusion protein can promote the production of AI-2.Optimum pH for AI-2 biosynthesis in vitro must be between 6-1 0.Biosynthesis of AI-2 is inhibited by sodium fluoride with final concentration of more than 0.3%.

Antibacterial effect of self-etching adhesive systems on Streptococcus mutans

OBJECTIVES: In this study, we evaluated the antibacterial activity of self-etching adhesive systems against Streptococcus mutans using the agar diffusion method. MATERIALS AND METHODS: Three 2-step systems, Clearfil SE Bond (SE, Kuraray), Contax (CT, DMG), and Unifil Bond (UnB, GC), and three 1-step systems, Easy Bond (EB, 3M ESPE), U-Bond (UB, Vericom), and All Bond SE (AB, BISCO) were used. 0.12% chlorhexidine (CHX, Bukwang) and 37% phosphoric acid gel (PA, Vericom) were used as positive controls. RESULTS: The antibacterial activity of CHX and PA was stronger than that of the other groups, except SE. After light activation, the inhibition zone was reduced in the case of all 2-step systems except CT. However, all 1-step systems did not exhibit any inhibition zone upon the light activation. CONCLUSIONS: SE may be better than CT or UnB among the 2-step systems with respect to antibacterial activity, however, 1-step systems do not exhibit any antibacterial activity after light curing.

Cuantificación de bacterias relacionadas con la caries dental en saliva de adultos y adultos mayores / Quantification of caries-associated bacteria from saliva of adults and older adults

Introducción: Se considera que Streptococcus mutans (S. mutans) y Lactobacillus spp. se asocian con la caries. Otras especies del biofilm oral, como Streptococcus sanguinis (S. sanguinis) han sido sindicadas como protectoras, pero principalmente en niños. Existe escasa evidencia sobre el nivel de estas bacterias en adultos mayores. Objetivo: Determinar si existen diferencias en los recuentos microbianos de tres especies relacionadas con la caries en pacientes adultos y adultos mayores. Materiales y Métodos: Una muestra de pacientes por conveniencia compuesta de 63 pacientes (18 a 79 años) proporcionó saliva estimulada con la que se sembraron placas de agar MSB, MM10 SB y Agar Rogosa para el cultivo de S. mutans, S. sanguinis y Lactobacillus spp., respectivamente. Los recuentos bacterianos fueron expresados como UFC/mL. Resultados: Los recuentos de S. mutans y Lactobacillus spp. no mostraron variaciones relacionadas con la edad (p>0.05). Los adultos mostraron mayores recuentos de S. sanguinis que los adultos mayores, 3.7 x 105 +/- 3.8 x 105 UFC/mL y 5.9 x 104 +/- 9.4 x 104 UFC/mL, respectivamente (p<0.05). Conclusiones: La edad no parece afectar los niveles de especies tradicionalmente consideradas como cariogénicas. Estos resultados sugieren que la edad puede relacionarse con los patrones de colonización de S. sanguinis en el biofilm oral.

Introduction: Streptococcus mutans (S. mutans) and Lactobacillus spp. have been traditionally associated with caries, regardless of the subject’s age. Other oral biofilm species have been linked as caries protective, including Streptococcus sanguinis (S. sanguinis), but mainly in children. Scarce evidence exists on the levels of these bacteria in older adults. Aim: To determine whether there are differences in the microbial counts of three caries-associated bacterial species in adults and older adults. Methodology: A convenience sample of sixty three patients, aged 18 to 79 years, participated in the study. Stimulated saliva samples were obtained and in MSB, MM10 and Rogosa agar plates for the culture of S. mutans, S. sanguinis and Lactobacillus spp., respectively. Bacterial counts were obtained by microscopic observation (10x) of the colonies and expressed in CFU/mL. Results: Bacterial counts of S. mutans and Lactobacillus spp. did not reveal age-related differences (p>0.05). Adults showed higher S. sanguinis counts than older adults with 3.7 x 105 +/- 3.8 x 105 CFU/mL and 5.9 x 104 +/- 9.4 x 104 CFU/mL, respectively (p<0.05). Conclusions: Age does not seem to affect the levels of bacterial species traditionally associated with caries. The results suggest that age may be related to colonization patterns of S. sanguinis in the oral biofilm.

Comparative evaluation of antibacterial property and substantivity of chlorhexidine containing dentifrices with sodium lauryl sulfate and Tween as surfactants: An in vivo study.

Aim: The aim of the study was to determine the antibacterial property and substantivity of chlorhexidine containing dentifrices with sodium lauryl sulfate (SLS) and Tween as surfactants. Materials and Methods: It is a double-blind cross over the study, a total of 20 children within their mixed dentition period (7-13 year) having Streptococci mutans count more than 106 were selected for the main study. Three types of chlorhexidine containing dentifrices were used with a washout period of 1 week. Out of the three toothpastes, one was without surfactant and other two toothpastes contained SLS and Tween as surfactants respectively. 20 volunteers brushed for 1 min during the study day with their assigned toothpaste. Saliva samples were collected before brushing, immediately after brushing and 1, 3, 5, and 7 hand sent for microbial analysis. The culture carried out by inoculating saliva sample onto Mitis salivarius agar for selective isolation of S. mutans followed by counting of colony forming unit. Results: Group I and III (Chlorhexidine and CHX + Tween) had shown statistically significant reduction in bacterial count until 7 h when compared to their baseline values (P < 0.001). Group II toothpaste (CHX + SLS) had shown significant reduction in bacterial count until 3 h only. On inter group comparison, Group III had shown good amount of percentage reduction in bacterial count when compared to other groups. Conclusion: CHX + Tween toothpaste had shown statistically significant reduction in antibacterial activity and substantivity than other groups. These findings show chlorhexidine containing toothpaste with non-ionic surfactant will be able to maintain the antibacterial property and substantivity of chlorhexidine.